Recombinant Human IgG4 Isotype Control
IsoC, WB, FACS, ELISA, ICC, IF, IP
Monoclonal
HyHEL-10
IgG4
Recombinant Antibody
unconjugated
Catalog No. ABIN7795388
Quick Overview for Recombinant Human IgG4 Isotype Control (ABIN7795388)
Target
IgG4
Antibody Type
Recombinant Antibody
Clonality
Monoclonal
Application
Isotype Control (IsoC), Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Clone
HyHEL-10
Isotype
IgG4, S228P
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Host
- Human
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Expression System
- Mammalian cells
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Purpose
- Recombinant Human IgG4 (S228P) Isotype Control Antibody
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Characteristics
- The anti-HEL monoclonal antibody (hen egg-white lysozyme) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant mouse IgG1 kappa isotype control (clone number: HyHEL-10) and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions. As an ideal recombinant mouse IgG1 isotype control, the recombinant HyHEL-10 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and has completely mouse variable regions and the mouse IgG1 kappa constant regions. The anti-HEL mouse IgG1 kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control. A variety of conjugates (such as dyes and fluorophores) with the anti-HEL isotype control and mutants are available.
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Purification
- Protein A or G affinity column
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Purity
- >95 % by reducing SDS-PAGE
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- > 3 mg/mL
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.
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- IgG4
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Alternative Name
- IgG4,S228P
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Target Type
- Antibody
Target
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